{"presentation":{"slides":[{"elements":[{"x":3.2679738562091503,"y":8.639308855291576,"width":46.84095860566449,"height":60.47516198704104,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

In Cartridge-based Nucleic Acid Amplification Test (CBNAAT) assay, the basis for detection of rifampicin resistance<\/strong><\/span> is the difference between the first or early Cycle threshold (Ct) and the last or late Ct value, of M. tuberculosis<\/em>-specific probes. Or the difference between probes with the maximum Ct value and minimum Ct value is greater than four.<\/p>\n\n

Rifampicin resistance not detected<\/strong><\/span>, will be declared in an MTB positive specimen when all the five probes are detected and the difference between probes with the maximum Ct value and minimum Ct value is less than four.<\/p>\n"},"subContentId":"32c7bb4a-5a97-4984-8a63-708d04badcea","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":52.287581699346404,"y":8.639308855291576,"width":44.989106753812635,"height":64.79481641468682,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b689ac875.png","mime":"image\/png","copyright":{"license":"U"},"width":545,"height":398}},"subContentId":"32134f5d-5127-4eeb-89b2-b8f6925bbe2c","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":51.19825708061002,"y":77.7537796976242,"width":47.930283224400874,"height":6.479481641468682,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Figure 1: Results interpretation for detection of RIF resistance<\/span><\/p>\n"},"subContentId":"0696c26d-bd0d-4dff-8907-b273abb6726e","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":3.2679738562091507,"y":6.491490280777537,"width":93.68191721132898,"height":69.11447084233261,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"

Complete Dropout and Delayed Ct<\/strong><\/p>\n\n

The molecular beacons are designed to hybridize with amplified wild-type, i.e., rifampicin sensitive, rpoB sequence.<\/p>\n\n

A mutation within this sequence will interfere with hybridization in such a way that the conformation of the probe, i.e., the molecular beacon may remain in the non-fluorescing state. Thus, a mutation anywhere in the core region of the rpoB gene will result in either:<\/p>\n\n

\n\t
Dropout:<\/strong> Complete suppression of fluorescence of the corresponding molecular beacon, resulting in a zero Ct value<\/li>\n\t
Delayed Ct value:<\/strong> Due to partial suppression<\/li>\n<\/ol>\n\n
Figures 2, 3 and 4 show these scenarios.<\/p>\n"},"subContentId":"600908e5-b2d6-44ef-9c68-f11713bd1c82","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":17.429193899782135,"y":8.639308855291576,"width":62.63616557734205,"height":73.43412526997841,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b721739c2.png","mime":"image\/png","copyright":{"license":"U"},"width":586,"height":348}},"subContentId":"06e70ec4-d4f1-4bc9-a67f-2bcdd7c18aec","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":15.250544662309368,"y":82.07343412526998,"width":70.80610021786492,"height":15.11879049676026,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 2: All MTB specific probes have CT values in a detectable range. The difference between the maximum and minimum CT values is < 4, therefore, the test is declared as RIF Resistance: Not Detected.<\/span><\/p>\n"},"subContentId":"730f6497-5b3e-4500-b641-da981699c02f","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":9.803921568627452,"y":8.639308855291576,"width":81.59041394335512,"height":71.27429805615552,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b7a0868a8.png","mime":"image\/png","copyright":{"license":"U"},"width":730,"height":323}},"subContentId":"56cfde58-cb2d-4514-af67-7a115692dc9f","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":29.411764705882355,"y":84.23326133909288,"width":45.751633986928105,"height":10.799136069114471,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 3: Probe E is a complete drop out showing a 0 Ct value, therefore, the result is declared as RIF Resistance detected.<\/span><\/p>\n"},"subContentId":"852f1be6-9ace-4ec0-bfcd-e2f15f291ead","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}},{"elements":[{"x":3.2679738562091503,"y":6.479481641468682,"width":62.091503267973856,"height":86.82505399568035,"action":{"library":"H5P.Image 1.1","params":{"decorative":false,"contentName":"Image","file":{"path":"images\/file-6435b7f5c7707.png","mime":"image\/png","copyright":{"license":"U"},"width":744,"height":527}},"subContentId":"19064d7e-1067-4268-ae83-8698fb215f81","metadata":{"contentType":"Image","license":"U","title":"Untitled Image"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""},{"x":67.53812636165577,"y":69.11447084233261,"width":30.501089324618736,"height":23.758099352051836,"action":{"library":"H5P.AdvancedText 1.1","params":{"text":"
Figure 4: Delayed Ct value for probe B. As the difference between maximum and minimum CT value is > 4, therefore, the result is declared as RIF resistance detected.<\/span><\/p>\n"},"subContentId":"df900ac5-12c6-4d0a-8674-b361a8a68d84","metadata":{"contentType":"Text","license":"U","title":"Untitled Text"}},"alwaysDisplayComments":false,"backgroundOpacity":0,"displayAsButton":false,"buttonSize":"big","goToSlideType":"specified","invisible":false,"solution":""}],"slideBackgroundSelector":{}}],"keywordListEnabled":true,"globalBackgroundSelector":{},"keywordListAlwaysShow":false,"keywordListAutoHide":false,"keywordListOpacity":90},"override":{"activeSurface":false,"hideSummarySlide":false,"summarySlideSolutionButton":true,"summarySlideRetryButton":true,"enablePrintButton":false,"social":{"showFacebookShare":false,"facebookShare":{"url":"@currentpageurl","quote":"I scored @score out of @maxScore on a task at @currentpageurl."},"showTwitterShare":false,"twitterShare":{"statement":"I scored @score out of @maxScore on a task at @currentpageurl.","url":"@currentpageurl","hashtags":"h5p, course"},"showGoogleShare":false,"googleShareUrl":"@currentpageurl"}},"l10n":{"slide":"Slide","score":"Score","yourScore":"Your Score","maxScore":"Max Score","total":"Total","totalScore":"Total Score","showSolutions":"Show solutions","retry":"Retry","exportAnswers":"Export text","hideKeywords":"Hide sidebar navigation menu","showKeywords":"Show sidebar navigation menu","fullscreen":"Fullscreen","exitFullscreen":"Exit fullscreen","prevSlide":"Previous slide","nextSlide":"Next slide","currentSlide":"Current slide","lastSlide":"Last slide","solutionModeTitle":"Exit solution mode","solutionModeText":"Solution Mode","summaryMultipleTaskText":"Multiple tasks","scoreMessage":"You achieved:","shareFacebook":"Share on Facebook","shareTwitter":"Share on Twitter","shareGoogle":"Share on Google+","summary":"Summary","solutionsButtonTitle":"Show comments","printTitle":"Print","printIngress":"How would you like to print this presentation?","printAllSlides":"Print all slides","printCurrentSlide":"Print current slide","noTitle":"No title","accessibilitySlideNavigationExplanation":"Use left and right arrow to change slide in that direction whenever canvas is selected.","accessibilityCanvasLabel":"Presentation canvas. Use left and right arrow to move between slides.","accessibilityProgressBarLabel":"Choose slide to display","containsNotCompleted":"@slideName contains not completed interaction","containsCompleted":"@slideName contains completed interaction","slideCount":"Slide @index of @total","containsOnlyCorrect":"@slideName only has correct answers","containsIncorrectAnswers":"@slideName has incorrect answers","shareResult":"Share Result","accessibilityTotalScore":"You got @score of @maxScore points in total","accessibilityEnteredFullscreen":"Entered fullscreen","accessibilityExitedFullscreen":"Exited fullscreen","confirmDialogHeader":"Submit your answers","confirmDialogText":"This will submit your results, do you want to continue?","confirmDialogConfirmText":"Submit and see results","slideshowNavigationLabel":"Slideshow navigation"}}

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Microbiologist (EQA)

Detection of MTB and RIF Resistance Results Interpretation in CBNAAT

Fluorescence Microscopy Using LED Microscope