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CDST_LT-M12:- Liquid culture

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  3. CDST_LT-M12:- Liquid culture
  4. CDST_LT-M12:- Liquid culture

  • CDST_LT: Introduction to MGIT 960 system

    • MGIT 960 Instrument: Operation

  • CDST_LT: Sample processing reagents preparation

    • Precautions for Sample Processing and Reagent Preparation in TB Culture Labs
    • BACTEC MGIT 960 Growth Supplement
    • Quality Control of MGIT 960 Tubes

  • CDST_LT: Specimen processing and inoculation

    • Specimen Processing for TB Cultures
    • Procedure for Culture Specimen Processing: Pulmonary specimens
    • Procedure for Culture Specimen Processing: Extrapulmonary specimens
    • Procedure for Culture Specimen Processing: Pus and other muco-purulent specimens
    • Procedure for Culture Specimen Processing: Gastric Aspirate
    • Procedure for Culture Specimen Processing: Bronchial Lavage
    • Procedure for Culture Specimen Processing: Laryngeal Swab
    • Procedure for Culture Specimen Processing: Tissue
    • Procedure for Culture Specimen Processing: Urine
    • Procedure for Culture Specimen Processing: CSF and other body fluids
    • Steps to be done after Culture Specimen Processing
    • Preparation of PANTA for MGIT TB Cultures
    • Preparation of MGIT Tubes for MGIT TB Cultures
    • Inoculation of MGIT Tubes
    • Incubation of MGIT Tubes

  • CDST_LT: Growth detection

    • BACTEC MGIT 960 System Growth Detection
    • Work-up for Positive MGIT Cultures
    • Interpreting MGIT Results

  • CDST_LT: Liquid culture contamination

    • Liquid Culture Contamination and sources of contamination
    • Monitoring Liquid Culture Contamination

  • CDST_LT: False culture results

    • False Positive MGIT Cultures
    • False Negative MGIT Cultures
    • Impact of False MGIT Culture Results

  • CDST_LT: Liquid culture troubleshooting

    • Troubleshooting LC Growth Recovery
    • Troubleshooting LC Detection Time
    • Troubleshooting High Liquid Culture contamination
    • Decontamination of Contaminated MGIT Culture

  • CDST_LT: MGIT Reading and reporting

    • Documentation of MGIT Cultures
    • MGIT Culture Reading Schedule
    • MGIT 960 Culture Detection Time Frame
    • MGIT 960 Instrument Reports
    • Reading MGIT 960 Tubes Manually

  • CDST_LT: Identification methods for MGIT 960 LC growth

    • LC Identification Methods for MTB/Isolated Mycobacteria
    • MTB growth characteristics and morphology
    • LC AFB Smears
    • Rapid Antigen Detection/Immunochromatographic Assay for MTB
    • Procedure for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
    • Inoculum Preparation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
    • Results interpretation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
    • Internal Quality Control Kits for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
    • Biochemical Tests for MTB
    • Susceptibility of MTB to p-Nitrobenzoic acid (PNB)
    • Niacin production test for MTB
    • Nitrate reduction test for MTB
    • Catalase peroxidase test for MTB

  • CDST_LT: Storage of culture isolates

    • Storage of Culture Isolates
    • Media and consumables required to store culture isolates
    • Preparation of Selective Middlebrook- 7H9 Liquid Medium
    • Quality Control of Selective Middlebrook- 7H9 Liquid Medium
    • Inoculation Procedure for Storage of Culture Isolates
    • Sterility Check of Culture Isolates
    • Incubation and Storage of Culture Isolates
    • Revival of the Frozen Cultures

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