Critical Concentrations and Reconstitution of SIRE Drugs

BACTEC MGIT 960 SIRE Kit for critical concentrations contains the following drugs in lyophilized form. Each kit contains one each of S, I, R, and E drug vial and 8 vials of MGIT 960 SIRE Supplement.

Drugs

Streptomycin - approximate lyophilized drug per vial --------------- 332µg

INH - approximate drug per vial ---------------------------------------- 33.2 µg

Rifampin – approximate drug per vial ---------------------------------- 332 µg

Ethambutol – approximate drug per vial -------------------------------1660 µg

SIRE supplement

The SIRE supplement vial differs from the MGIT Growth Supplement and contains, per litre of purified water, the following:

Bovine albumin -------------------- 50.0 g

Dextrose----------------------------- 20.0 g

Catalase ----------------------------- 0.03 g

Oleic acid ---------------------------- 0.6 g

 Storage

Upon receipt, refrigerate the lyophilized drugs at 2-8ºC. Reconstitute prior to use. Once opened and reconstituted, the leftover drug solutions may be frozen in aliquots at -20ºC or lower and can be stored for up to 6 months or up to the date of original expiry, whichever comes sooner. Once thawed, discard the leftover and do not store or refreeze.    

Reconstitution of SIRE Drug Lyophilized Drugs (stock preparation)

Reconstitute the drugs with the appropriate volume of diluent as described below. Volumes vary with different drugs. Failure to use the appropriate volume will invalidate these tests.

Preparation of drugs in MGIT 960 SIRE Kit

1 Reconstitute each Streptomycin lyophilized drug vial with 4 ml of sterile distilled/ deionized water to make a stock solution of 83μg/ml.

2 Reconstitute each Isoniazid lyophilized drug vial with 4 ml of sterile distilled/ deionized water to make a stock solution of 8.3μg/ml.

3 Reconstitute each Rifampicin lyophilized drug vial with 4 ml of sterile distilled/ deionized water to make a stock solution of 83μg/ml.

4 Reconstitute each Ethambutol lyophilized drug vial with 4 ml of sterile distilled/ deionized water to make a stock solution of 415μg/ml.

Resource

Mycobacteriology Laboratory Manual       

Assessment