Media and consumables required to store culture isolates

 MTB isolates from all positive cultures (using the LJ subculture from a MGIT tube positive for MTB or a subculture from the original LJ culture, if MGIT is unavailable) will be frozen in 7H9 broth and glycerol to preserve them for any repeat or additional microbiology tests that may need to be performed (either in-house or at a central laboratory). It is recommended that at least four aliquots from baseline visits, and two aliquots from subsequent visits, be frozen for each MTB isolate.

• Media and consumables required to store culture isolates

  1. Positive LJ slants (Solid Culture: Lowenstein Jensen (LJ) Media)

   2.2 ml sterile cryotubes with a screw top, externally threaded

  3. Water bath

  4.7H9 media with glycerol

  5. ADC or OADC enrichment broth

  6. Sterile transfer pipettes with graduations marking volume (individually wrapped)

  7. Sterile loop, disposable applicator stick, or cotton swab

  8. Permanent marker

  9. Cryobox and rack

  10. Study-specific labels

     Resource

    https://stoptb.org/wg/gli/assets/documents/gli_mycobacteriology_lab_manual_web.pdf        

     Assessment