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Consumables required at a Microscopy Centre
Learning ObjectivesList out the consumables which are required at the DMC including, reagents, chemicals, sputum cups, oils, paper tissues, sticks etc. Also include specifications for each
The consumables may be grouped under the different headings( if specific function like staining, slide preparation, logistics, etc )
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- Microscopy Centres (DMCs) are the most peripheral laboratory under the National TB Elimination Programme (NTEP) network.
- Any person identified to be a presumptive TB patient is first referred to the nearest DMC for sputum examination or their sputum specimens are collected and transported to the DMC. Therefore, it is very important for the DMCs to maintain an adequate stock of reagents and other consumables.
- The Medical Officer (MO) of DMC is responsible for determining the number of reagents and other materials the DMC needs every month.
- The Senior Tuberculosis Laboratory Supervisor (STLS) ensures these supplies are distributed in a timely manner, as and when required.
- The Lab Technician (LT) is responsible for exhausting the old supplies before the new ones.
The consumables required at the DMC can be broadly categorised as:
- Consumables required for sputum collection
- Consumables required for slide preparation
- Consumables required for smear examination
- Consumables stationery
- Other
| Table: List of Items with Technical Specifications of Laboratory Consumables Required for DMCs | ||
| Sl. No. | Name of the item | Technical Specifications |
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| Sputum containers | Cups made of Special Medical Grade Polypropylene, thin plastic, translucent, diameter - 4 cm, capacity - 30 ml, the screwable cap should also be made of Special Medical Grade Polypropylene and should be airtight and leak-proof. | |
| Absorbent cotton | 500 gms/roll | |
| Phenolic compound | 5% phenol/ 40% phenolic compound (proprietary Phenyl) diluted to 5% | |
| Adhesive labels for sputum containers | ||
| Sputum specimen transport box | Insulated box, made of plastic 10” x 10” x 10”, thickness 1” with lid, handle and nylon belt 1” width 2.5 feet length, nylon strap of 1” width 2 feet length with velcro to strap the lid of the box from side to side. | |
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| Slides for Microscopes | Glass slides plain, size = 76mm x 26mm x 1.3mm, clean, scratch-free with smooth edges, uniform refractive index, pack of 50. | |
| Broomstick | 10 cms in length and thick enough to make good smears. | |
| Diamond marker pencil | 6" (15.24 cm.) holder with artificial diamond (hard stone) embedded at one end with a screw cap, to mark on microscope glass slides. | |
| Grease marking pencil | Marking pencil MPS, blue or red coloured, 8" length, to write on glassware/ metal surfaces. | |
| Staining racks | For drying the slides. | |
| Slide boxes for storing slides | ||
| Glass (or metal) rods | For holding slides during the staining process. | |
| Forceps, Chitel forceps | Stainless steel for slides, 15 cm. | |
| Scissors | 25 cm, stainless steel | |
| Ziehl Neelsen stain | ||
| Auramine O fluorescent dye for Fluorescence Microscopy (FM) | Wherever FM is being done | |
| Whatman filter paper No 1 | ||
| 3 ) For smear examination | ||
| Binocular Microscope | With 10x, 40x and oil immersion objective (100x) eyepieces (10x) and spare bulbs and fuses. | |
| Immersion oil | ||
| Filter paper | To drain the oil from the slides. | |
| Fine Silk and Lint cloth | ||
| Lens paper | For wiping the oil immersion lens after examination of each slide | |
| 4) Consumables - Stationery | ||
| Request form for examination of biological specimen for sputum examination | ||
| TB Laboratory Register | ||
| Referral/ Transfer form for treatment | ||
| Stock register – laboratory | ||
| Marker pen | ||
| 5 ) Other | ||
| Plastic tumblers/ mugs | ||
| Time (stop-watch) | ||
| Spirit lamp or Bunsen burner | ||
| Silica gel | Hygroscopic agent to maintain the microscope in a moisture-free environment (to be placed in the cabinet for Binocular Microscope). | |
| Alcohol (absolute) | Ethanol | |
| Cotton, full sleeves Aprons | ||
| Disposable gloves | ||
| Methylated spirit |
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Resources
- Training Module (1-4) for Program Managers and Medical Officers, NTEP, MoHFW, 2020.
- Guidelines for Quality Assurance of Smear Microscopy for Diagnosing Tuberculosis, RNTCP Lab Network, CTD, MoHFW, India, 2005.
- Module for Laboratory Technicians, CTD, MoHFW, India, 2005.
Assessment
| Question | Answer 1 | Answer 2 | Answer 3 | Answer 4 | Correct answer | Correct explanation | Page id | Part of Pre-test | Part of Post-test |
| Sputum containers and their screwable cap used for sputum collection at the DMC should be made of Special Medical Grade Polypropylene and should be air-tight. | True | False | 1 | Sputum containers and their screwable cap used for sputum collection at the DMC should be made of Special Medical Grade Polypropylene and should be air-tight and leakproof. | | Yes | Yes | ||
| Who ensures that supplies are distributed in a timely manner at DMCs? | Treatment Supporter | Medical Officer | Senior Tuberculosis Laboratory Supervisor (STLS) | Lab Technician (LT) | 3 |
The Senior Tuberculosis Laboratory Supervisor (STLS) ensures these supplies are distributed in a timely manner, as and when required. |
Yes | Yes |
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