Overview of the Truenat Testing Process

The Truenat Assay Technology works on Real-time Polymerase Chain Reaction (PCR).

The key steps include:

1. Collection of specimens from a presumptive TB/known TB Patient (for UDST).
2. Liquefaction and lysis of specimen using the Trueprep AUTO MTB sample pre-treatment pack (20 minutes)
3. Extraction and purification of DNA using Trueprep AUTO v2 Universal Cartridge-based sample prep kit and Trueprep AUTO v2 Universal Cartridge-based sample prep device (20 minutes)
4. Amplification of extracted DNA by the Truelab Real-time micro-PCR analyzer using freeze dry PCR reagent in microtubes on a Truenat MTB chip (35 minutes)
5. DNA from positive test results is tested using Truenat MTB RIF Dx chip as a reflex test (55 minutes)

The process flow from sample to result for Truenat Assay Technology is described in the figure below.

Figure: Process flow from sample to result for Truenat Assay Technology; Source: Practical Guide to Implementation of Truenat™ Tests for the Detection of TB and Rifampicin Resistance.

Resources

• Truenat MTB Kit Insert.
• Trueprep® AUTO Universal Cartridge-based Sample Prep Device.
• Practical Guide to Implementation of Truenat™ Tests for the Detection of TB and Rifampicin Resistance.

Kindly provide your valuable feedback on the page to the link provided HERE