Limitations of Second-line Line Probe Assay [SL-LPA]

The limitations of Second Line Probe Assay (SL-LPA) are:

• It detects resistance due to mutation in gyrA, gyrB, rrs, and eis genes; resistances originating from mutations of other genes or gene regions as well as other drug resistance cannot be determined.
• Individual strains in mixed samples cannot be differentiated, which hampers the interpretation of the test.
• It cannot be used to monitor drug treatment, as DNA is detected from viable and non-viable bacteria.
• The results are qualitative, the intensity of bands does not give an estimate of bacterial load in the sample.

Resources

• GenoType MTBDRsl Ver 2.0 Kit, Instructions for Use.

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