Interpretation of SL-LPA: Interpretation Examples

he examples of banding patterns for Second Line - Line Probe Assay (SL - LPA) are shown in the figure below.

Description

• All wild type bands display signal, mark as “+” against Wild Type (WT) column of the respective gene.
• If at least one of the wild type bands is absent, mark as “-“ against WT column. Similarly, “+” or “-” for Mutation probes (MUT).

Figure: Examples for banding patterns for SL - LPA; Source: GenoType MTBDRsl Ver 2.0 Kit, Instructions for Use.

Example #1

• The wild type banding pattern.
• All wild type probes, but none of the mutation probes display a signal; hence, evaluation chart shows “+”.
• Four wild type columns and “–” in the four mutation columns; hence, no entry made in the fields of resistance columns.

Example #7

• gyrA locus and gyrB locus show wild type pattern; hence, evaluation chart shows “+” for fluoroquinolone (ofloxacin) resistance.
• rrs locus shows wild type pattern; hence, evaluation chart shows “+” for Kanamycin.
• eis wild type bands are present and “eis MUT” is absent; low-level Kanamycin resistance is assigned to the test.

Resources

• GenoType MTBDRsl Ver 2.0 Kit, Instructions for Use.

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