Contamination Control in the Hybridization Area in LPA Lab Settings

Do’s and Don’ts for Contamination Control 

DO’s

• Label the tools and instruments “For use in Hybridization Area”.​
• Change gloves if they get contaminated amplicon.​
• Decontaminate trays before reuse​.
• Keep cleaned strip trays and tweezers in a clean and sealed plastic bag.​
• Always clean work bench and tools with 1% freshly prepared sodium hypochlorite solution followed by 70% alcohol​.
• Establish weekly laboratory cleaning protocols for floors, doors, walls.

DON’Ts

• Tools and instruments used in hybridization area should not be used elsewhere​.
• Never take anything from hybridization area to reagent or specimen preparation area​.
• Do not reuse falcon tubes for conjugate and substrate preparation.​
• Do not decant the content of the strip tray into the sink or any other container during the Hybridisation (HYB) and Stringent (STR) steps. This will contaminate the work area​.

Resources

• GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
• GenoType MTBDRsl VER 2.0 kit, Instructions for Use.

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