Extraction of DNA from Smear Positive Clinical Specimen, LJ and Liquid Media in LPA Lab Settings

Purpose of DNA extraction 

• Extract DNA from cellular components 

For Line Probe Assay (LPA), DNA is extracted from:  

• Clinical specimen​ 
• Culture isolates on Loewenstein-Jensen (LJ) solid medium  
• Bacteria growth in liquid media​ (Mycobacteria Growth Indicator Tube (MGIT), BD Diagnostics) 

Extraction of DNA from a clinical specimen and culture isolates (LJ, liquid) is shown in the figure below: 

Figure: Extraction of DNA from a Clinical Specimen and Culture Isolates 

Resources 

• Guidelines for PMDT in India, 2021. 
• Challenge TB: LPA - Introduction to Molecular Biology​.
• GenoType MTBDR plus ver 2.0 kit, Instructions for Use. 

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