LPA Laboratory Equipment: Twincubator

Uses of Twincubator in Line Probe Assay (LPA):

• Different steps of the hybridization (shown in figure below) and detection procedure with DNA strips in reverse blot hybridization assays.
• In the amplification/ post-amplification area.

Figure: Twincubator used in Hybridization

Features of Twincubator: ​

• Provides dry incubation​
• Thermo-shaker​
• Programmable​
• Temperature and time-controlled features​
• Pre-installed temperature profile for processing of the LPA tests​
• Nine storage programs with up to 20 steps​
• No. of samples run - 1 to 12, including one positive and two negative controls​
• User friendly with minimum risk of contamination​

Note: Always refer to the user manual for operation​.

Maintenance:

• Always switch off the appliance before any activity​.
• Always record every maintenance activity performed​.
• Clean the housing of the instrument​.
• The instrument must be protected from harsh chemicals​.
• Clean the instrument immediately, if liquid enters the hybridization block.
• Perform preventive maintenance (temperature check) regularly​.

Resources

• Molecular Detection of Drug-resistant Tuberculosis By Line Probe Assay

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