LPA Laboratory Equipment: Water Bath

In the Line Probe Assay (LPA) lab, the water bath is used for warming reagents used in the Hybridization Room.

It is electrically heated and electronically controlled.

Components:

• Recessing push/ turn control for simple operation of the bath
• Visual temperature indication​
• Push button to set desired temperature​
• Electrically heated and electronically controlled​
• Water trough​

Installation of Water Bath (Figure):

• The unit must be placed on a horizontal, non-flammable sturdy surface. ​​
• The vent openings must remain unobstructed. ​​
• Minimum wall spacing on all sides is 80 millimetres. ​​
• The minimum spacing from top of the bath to the next ceiling must be 750 millimetres. ​

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Figure: Spacing during Installation of Water Bath (Front and Side View)

Working with the Water Bath: ​

The heating positioned on three sides around the tank ensures a natural water circulation of the liquid inside, thus securing an optimal uniform temperature distribution​.

• Vent openings must be unobstructed​.
• The surface must be horizontal, nonflammable during installation​.

General Instructions for Maintenance

• Always switch off the appliance before any maintenance activity​.
• Always record the maintenance activity performed​.
• Rusting objects must not come into contact with stainless steel bath tank​.
• Rusting areas must be cleaned and polished immediately​.
• Lubricate/ oil the hinge bolts from time to time​.
• Replace distilled/ demineralized water and clean the water bath every week​.

Annual Maintenance

• This is done by a qualified service technician
• Includes the calibration or validation of the temperature with a thermostat/ digital controller with devices traceable to National/ International Standards​.

Resources

• Molecular Detection of Drug-Resistant Tuberculosis by Line Probe Assay.

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