Inoculation of Brain Heart Infusion [BHI] Agar

Brain Heart Infusion (BHI) Agar is a general-purpose medium​ that is:

• Prepared using dehydrated ingredients
• Suitable to grow bacteria, yeasts and moulds​
• ​Used for reagent quality control and contamination check for liquid culture​.

To check contamination in Mycobacteria Growth Indicator Tube (MGIT) broth:

• Visually inspect MGIT tubes for heavy turbidity. Heavy turbidity indicates contamination as Mycobacterium tuberculosis growth appears in form of particles without any significant turbidity.
• Divide the BHI agar plate into four quadrants, sub-culture loopful of turbid growth from MGIT tubes in each quadrant (small streak for each specimen, properly labelled).
• Incubate at 37ºC and observe after 24-48 hours for growth of contaminants.

To check the sterility of reagents/ processed specimens:

• Spread one drop of reagent/ sediment on BHI plate​.
• Incubate the inoculated BHI plates at 37℃ for 24-48 hours to check for contaminants​.

Resources

• MGIT Procedure Manual.

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