Precautions for Sample Processing and Reagent Preparation in TB Culture Labs

Precautions for sample processing and reagent preparation in TB culture labs are as follows:

• Precaution is necessary while performing aerosol-generating procedures such as centrifugation, vertexing, mixing, pipetting, pouring and inoculation of media. For example -delay the opening of caps until aerosols have settled, open centrifuge canisters only inside the Bio Safety Cabinets (BSCs), use pipettes that are easy to control.
• Safety precautions to minimize interruption of airflow inside the BSC by keeping arms parallel to the work surface inside the BSC and working in the center to minimize arm movements and ensure not to move hands out of the hood until work is completed. Minimize the equipment inside the BSC so that there is no interference in the airflow pattern.
• Disinfect the BSC and all work surfaces with a tuberculocidal disinfectant before and after every procedure.
• The autoclave should be monitored with a spore test, at least monthly, to ensure that sterility is achieved.
• Avoid practices that can result in spills.
• Train all personnel working in the TB culture lab.
• Working staff must wear Personal Protective Equipment (PPE) like protective gowns, gloves, hair covers and shoe covers etc.

Resources

• Mycobacteriology Laboratory Manual, Stop TB Partnership, 2014.

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